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anti bubr1 rabbit antibody  (Bethyl)


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    Structured Review

    Bethyl anti bubr1 rabbit antibody
    Anti Bubr1 Rabbit Antibody, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 138 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bubr1+a300+386a/BubR1+Antibody/bio_rxiv__2025__07__25__666731-190-27-31
    Average 93 stars, based on 138 article reviews
    anti bubr1 rabbit antibody - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Western Blot:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..

    Immunofluorescence:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..

    Immunoprecipitation:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..

    Clone Assay:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..

    Generated:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..

    Affinity Purification:

    Article Title: The internal Cdc20 binding site in BubR1 facilitates both spindle assembly checkpoint signalling and silencing.
    Article Snippet: The generation of stable HeLa cell lines was done using the Flp-In system (Invitrogen) and the resulting clones were kept under selection by supplementing the growth media with 200 mg ml 1 Hygromycin B and 5 mg ml 1 Blasticidin S. Antibodies. .. The following antibodies were used for western blot, immunofluorescence or immunoprecipitation as indicated: a-tubulin 11H10 (rabbit, Cell Signaling) 1:100 IF, b-actin AC-15 (mouse, Abcam) 1:5,000 WB, APC1 A301– 653A (rabbit, Bethyl) 1:500 WB, APC3 35/CDC27 (mouse, BD Biosciences) 1:250 WB, APC4 (mouse, raised against a C-terminal peptide) 5 mg IP, APC7 A302–551A (rabbit, Bethyl) 1:1,000 WB, Bub3 clone 31 (mouse, BD Transduction Laboratories) 1:500 WB, BubR1 A300–995A (rabbit, Bethyl) 1:500 WB, BubR1 A300–386A (rabbit, Bethyl) 1:200 IF, Cdc20 AR12 (mouse, Millipore) 1:200 IF/5 mg IP, Cdc20 E-7 (mouse, Santa Cruz Biotechnology) 1:500 WB, Cdc20 A301–180A (rabbit, Bethyl) 1:1,000 WB, CREST (human, Antibodies Inc.) 1:400 IF, FLAG M2 (mouse, Sigma) 1:5,000 WB, GFP clones 7.1 and 13.1 (mouse, Roche) 1:5,000 WB /1:200 IF, Mad2 from G. Kops (rabbit) 1:200 IF, Mad2 A300–301A (rabbit, Bethyl) 1:1,000 WB, p31 rabbit antibody was generated using full-length p31 as antigen and affinity purified. ..



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